Moldovan F, Soliman H R, Bennani H, Dumas J, Prévost D, Cussenot O, Mazières C, Le Brun G, Darbord C, Fiet J
Laboratoire de biologie hormonale, hôpital Saint-Louis, Paris, France.
C R Acad Sci III. 1995 Sep;318(9):951-8.
An immortalized human endothelial cell line was obtained by transfecting umbilical vein endothelial cells in primary culture with plasmid pMK16 containing SV40 replicated origin defective gene. The essential functional properties demonstrated in these immortalized human endothelial cells also retaining the classical phenotypical characteristics of endothelial cells in primary culture are: (1) endothelin-1 secretion; (2) capacity to convert big endothelin-1 into endothelin-1; (3) the capacity to secrete IL1 beta and IL6 interleukins both spontaneously and after lipopolysaccharide (LPS) stimulation; (4) arginine transfer from the extracellular to the intracellular medium. Such stable cell line could facilitate studies of regulation of endothelin-1 production; (5) No-synthase activity; (6) binding and metabolisation of acetylated low-density lipoproteins.
通过用含有SV40复制起点缺陷基因的质粒pMK16转染原代培养的脐静脉内皮细胞，获得了一种永生化的人内皮细胞系。这些永生化的人内皮细胞所表现出的基本功能特性，同时还保留了原代培养内皮细胞的经典表型特征，包括：（1）内皮素-1分泌；（2）将大内皮素-1转化为内皮素-1的能力；（3）自发分泌以及在脂多糖（LPS）刺激后分泌IL1β和IL6白细胞介素的能力；（4）精氨酸从细胞外介质转移到细胞内介质。这种稳定的细胞系有助于研究内皮素-1产生的调节；（5）无合酶活性；（6）乙酰化低密度脂蛋白的结合和代谢。
