Sudo K, Takahashi E, Nakamura Y
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Cytogenet Cell Genet. 1995;71(4):385-8. doi: 10.1159/000134145.
We report isolation of human cDNA highly homologous to murine Eif4a2, a gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome. This cDNA, which encodes a 407-amino-acid protein, belongs to a highly-conserved gene family, the DEAD-box gene family. The human homologue of Eif4a2 was expressed in all normal tissues examined, but in variable amounts, being highly expressed in skeletal muscle and ovary, and less abundantly in liver, kidney, and pancreas. Furthermore, we have localized the human EIF4A2 to chromosome 18p11.2 by fluorescent in situ hybridization.
我们报告了与小鼠Eif4a2高度同源的人cDNA的分离，Eif4a2是一种参与mRNA与核糖体结合的蛋白质合成起始因子的基因。该cDNA编码一个407个氨基酸的蛋白质，属于一个高度保守的基因家族——DEAD-box基因家族。Eif4a2的人类同源物在所有检测的正常组织中均有表达，但表达量各不相同，在骨骼肌和卵巢中高表达，在肝脏、肾脏和胰腺中表达较少。此外，我们通过荧光原位杂交将人类EIF4A2定位于染色体18p11.2。
