Ebisuno S, Kohjimoto Y, Tamura M, Ohkawa T
Department of Urology, Minami Wakayama National Hospital, Japan.
Eur Urol. 1995;28(1):68-73. doi: 10.1159/000475023.
The present investigation studied the quantitative adhesion of calcium oxalate monohydrate (COM) crystals to the surface of Madin-Darby canine kidney cells, which exhibit many characteristics of renal cortical collecting tubule cells. COM crystals adhered to the cell surface, and the attachment showed a time and concentration dependency with plateau. The results suggested that the attachment of microcrystals to the cortical tubular cell might be one of the earliest processes in the formation of kidney stones. Pretreatment with glycosaminoglycans significantly reduced the adherent crystals. Injuries to the Madin-Darby cells induced by 0.1 M HCl and gentamicin resulted in significant decreases of COM crystal adhesion to the cell surface. It was suggested that urinary glycosaminoglycans might play some critical role in preventing crystal adhesion to these cellular membranes and that cell injuries might not be essential for the attachment of microcrystals to the tubular cells.
本研究探讨了一水合草酸钙（COM）晶体与马-达二氏犬肾细胞表面的定量黏附情况，该细胞呈现出肾皮质集合管细胞的许多特征。COM晶体黏附于细胞表面，且这种黏附表现出时间和浓度依赖性并趋于平稳。结果表明，微晶黏附于皮质肾小管细胞可能是肾结石形成的最早过程之一。用糖胺聚糖预处理可显著减少黏附的晶体。0.1 M盐酸和庆大霉素对马-达二氏细胞造成的损伤导致COM晶体对细胞表面的黏附显著减少。研究表明，尿糖胺聚糖可能在防止晶体黏附于这些细胞膜方面发挥关键作用，并且细胞损伤对于微晶黏附于肾小管细胞可能并非必不可少。
