Waldmann T, Nitsch M, Klumpp M, Baumeister W
Max Planck Institut für Biochemie, Martinsried, Germany.
FEBS Lett. 1995 Nov 27;376(1-2):67-73. doi: 10.1016/0014-5793(95)01248-8.
Co-expression of the two genes encoding the alpha- and beta-subunit of the Thermoplasma acidophilum thermosome in Escherichia coli yielded fully assembled hetero-oligomeric complexes (alpha+beta). Surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (alpha, beta) in the bacterial cytoplasm. On electron micrographs these complexes were indistinguishable from each other and from the native thermosome. The recombinant alpha-complex as well as the native thermosome could be reconstituted in vitro from their dissociated subunits in the presence of Mg-ATP.
嗜热栖热菌热体α亚基和β亚基的两个编码基因在大肠杆菌中共同表达产生了完全组装的异源寡聚复合物（α+β）。令人惊讶的是，两个基因单独表达也导致在细菌细胞质中形成十六聚体复合物（α，β）。在电子显微镜照片上，这些复合物彼此之间以及与天然热体无法区分。重组α复合物以及天然热体可以在Mg-ATP存在的情况下从其解离的亚基在体外进行重构。
