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针对裂殖酵母tms1蛋白产生的三种单克隆抗体的精确表位图谱分析

Precise epitope mapping of three monoclonal antibodies raised against tms1 protein of fission yeast.

作者信息

Schneider E, Fuchs A, Nastainczyk W, Montenarh M, Wagner P

机构信息

Medical Biochemistry, University of Saarland, Homburg/Saar, Germany.

出版信息

Hybridoma. 1995 Aug;14(4):329-33. doi: 10.1089/hyb.1995.14.329.

DOI:10.1089/hyb.1995.14.329
PMID:8522343
Abstract

Recently we described the production of two monoclonal antibodies 10G2 and 10C4 of IgG3 subclass raised against the recombinant tms1 protein of fission yeast. Here we introduce a new monoclonal antibody 2E2 of IgG1 subclass and present the precise epitope mapping of these monoclonal antibodies using tms1 deletion mutants and synthetically produced oligopeptides spanning the tms1 protein by immunoblot analysis.

摘要

最近，我们描述了针对裂殖酵母重组tms1蛋白产生的两种IgG3亚类单克隆抗体10G2和10C4。在此，我们介绍一种新的IgG1亚类单克隆抗体2E2，并通过免疫印迹分析，利用tms1缺失突变体和跨越tms1蛋白的合成寡肽，对这些单克隆抗体进行精确的表位定位。

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