Moons L, D'Hondt E, Pijcke K, Vandesande F
Laboratory of Neuroendocrinology, Zoological Institute, Leuven, Belgium.
J Comp Neurol. 1995 Sep 18;360(2):331-48. doi: 10.1002/cne.903600210.
A light microscopic immunocytochemical study, using antisera against noradrenaline (NA) and dopamine-beta-hydroxylase (DBH), revealed the noradrenergic system in the brain of the chicken (Gallus domesticus). NA- and DBH-immunoreactive (ir) elements showed a similar distribution throughout the whole brain. The neurons immunoreactive for the monoamine were confined to the lower brainstem, the pons, and the medulla. In the pons, a rather dense group of cells was found in the dorsal, most posterior part of the locus coeruleus and in the caudal nucleus subcoeruleus ventralis. A few labeled cells appeared in and around the nucleus olivaris superior in the most caudal part of the metencephalic tegmentum. In the medulla oblongata, noradrenergic cells could be visualized at the level of the nucleus of the solitary tract and in a ventrolateral complex. Virtually all regions of the brain contained a rather dense innervation by NA- and DBH-immunopositive varicose fibers. Noradrenergic fibers and terminals were especially abundant in the ventral forebrain and in the periventricular hypothalamic regions. DBH-ir and NA-ir fibers, varicosities, and punctate structures could be observed in close association with immunonegative perikarya in several brain regions, more specifically in the ventral telencephalon, in the mid- and tuberal hypothalamic region, and in the dorsal rostral pons. Some perikarya in these brain areas were completely surrounded by noradrenergic structures that formed pericellular arrangements around the cells. The present study on the distribution of the noradrenergic system in the brain of the chicken combined with the results of a previous report on the distribution of L-Dopa and dopamine in the same species (L. Moons, J. van Gils, E. Ghijsels, and F. Vandesande, 1994, J. Comp. Neurol. 346:97-118) offers the opportunity to differentiate between the various catecholamines in the brain of this vertebrate. The results are discussed in relation to catecholaminergic systems previously reported in avian species and in the mammalian brain.
一项使用抗去甲肾上腺素（NA）和多巴胺-β-羟化酶（DBH）抗血清的光学显微镜免疫细胞化学研究，揭示了家鸡（Gallus domesticus）脑中的去甲肾上腺素能系统。NA免疫反应性（ir）和DBH免疫反应性元素在整个大脑中显示出相似的分布。对单胺免疫反应的神经元局限于脑桥下部、脑桥和延髓。在脑桥中，在蓝斑最背侧、最后部以及尾侧蓝斑下核腹侧发现了一组相当密集的细胞。在脑桥后脑被盖最尾端的上橄榄核及其周围出现了一些标记细胞。在延髓，在孤束核水平和腹外侧复合体中可以看到去甲肾上腺素能细胞。实际上，大脑的几乎所有区域都有相当密集的由NA和DBH免疫阳性曲张纤维组成的神经支配。去甲肾上腺素能纤维和终末在腹侧前脑和室周下丘脑区域特别丰富。在几个脑区，特别是在腹侧端脑、下丘脑中部和结节部区域以及脑桥背侧吻端，可以观察到DBH免疫反应性和NA免疫反应性纤维、曲张体和点状结构与免疫阴性的核周体紧密相连。这些脑区的一些核周体完全被形成围绕细胞的细胞周排列的去甲肾上腺素能结构所包围。本研究关于家鸡脑中去甲肾上腺素能系统的分布，结合先前关于同一物种中左旋多巴和多巴胺分布的报告结果（L. Moons、J. van Gils、E. Ghijsels和F. Vandesande，1994，《比较神经学杂志》346:97 - 118），提供了区分该脊椎动物脑中各种儿茶酚胺的机会。结果结合先前在鸟类和哺乳动物脑中报道的儿茶酚胺能系统进行了讨论。
