Pauwels P J, Wurch T, Amoureux M C, Palmier C, Colpaert F C
Laboratory of Cellular and Molecular Neurobiology, Centre de Recherche Pierre Fabre, Castres, France.
J Neurochem. 1996 Jan;66(1):65-73. doi: 10.1046/j.1471-4159.1996.66010065.x.
The involvement of serotonin 5-HT1D beta receptor sites was investigated in the growth of rat C6 glial cells permanently transfected with a gene encoding a human 5-HT1D beta receptor. The 5-HT receptor identity of control and transfected C6 glial/5-HT1D beta cells was determined by reverse transcription-polymerase chain reaction using primers specific for rat 5-HT1A, rat 5-HT1B, rat 5-HT1D alpha, human 5-HT1D beta, and rat 5-HT2A receptor genes. Constitutive mRNA for 5-HT2A receptors was present in control and transfected C6 glial/5-HT1D beta cells, whereas mRNA for 5-HT1D beta receptor sites was only present in the transfected C6 glial/5-HT1D beta cell line. 5-HT inhibited forskolin-stimulated cyclic AMP formation and promoted cell growth, in contrast to the absence of any measurable effect in pcDNA3 plasmid-transfected and nontransfected C6 glial cells. The 5-HT effects could be mimicked by sumatriptan (EC50 = 44-76 nM) and were totally and partially blocked by methiothepin (IC50 = 9 nM) and GR 127,935 (2'-methyl-4'-(5-methyl[1,2,4]oxadiazol-3-yl)-biphenyl-4-carbox yli c acid [4-methoxy-3-(4-methylpiperazin-1-yl)phenyl]amide; IC50 = 97 pM), respectively. No effect on cell growth was measured with the 5-HT2 receptor agonist DOI [1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane; 10 microM], suggesting that 5-HT2A receptors are not involved in the 5-HT-stimulated C6 glial/5-HT1D beta cell growth. Dibutyryl-cyclic AMP (0.3 mM)-treated cultures did not show sumatriptan-promoted cell growth, indicating an inhibitory role for cyclic AMP in the cell growth mediated by 5-HT1D beta receptor sites.(ABSTRACT TRUNCATED AT 250 WORDS)
在永久转染了编码人5-羟色胺1Dβ受体基因的大鼠C6神经胶质细胞生长过程中，对5-羟色胺5-HT1Dβ受体位点的参与情况进行了研究。使用针对大鼠5-HT1A、大鼠5-HT1B、大鼠5-HT1Dα、人5-HT1Dβ和大鼠5-HT2A受体基因的引物，通过逆转录-聚合酶链反应确定对照和转染的C6神经胶质/5-HT1Dβ细胞的5-羟色胺受体特性。5-HT2A受体的组成型mRNA存在于对照和转染的C6神经胶质/5-HT1Dβ细胞中，而5-HT1Dβ受体位点的mRNA仅存在于转染的C6神经胶质/5-HT1Dβ细胞系中。与在pcDNA3质粒转染和未转染的C6神经胶质细胞中未观察到任何可测量的效应相反，5-羟色胺抑制了福司可林刺激的环磷酸腺苷形成并促进了细胞生长。舒马曲坦（EC50 = 44-76 nM）可模拟5-羟色胺的作用，甲硫噻平（IC50 = 9 nM）和GR 127,935（2'-甲基-4'-(5-甲基[1,2,4]恶二唑-3-基)-联苯-4-羧酸[4-甲氧基-3-(4-甲基哌嗪-1-基)苯基]酰胺；IC50 = 97 pM）分别完全和部分阻断该作用。5-HT2受体激动剂DOI [1-(2,5-二甲氧基-4-碘苯基)-2-氨基丙烷；10 μM]对细胞生长无影响，表明5-HT2A受体不参与5-羟色胺刺激的C6神经胶质/5-HT1Dβ细胞生长。用二丁酰环磷酸腺苷（0.3 mM）处理的培养物未显示舒马曲坦促进的细胞生长，表明环磷酸腺苷在5-HT1Dβ受体位点介导的细胞生长中起抑制作用。（摘要截短于250字）
