Morishita A
Department of Otolaryngology, Kuwana Municipal Hospital.
Nihon Jibiinkoka Gakkai Kaiho. 1995 Sep;98(9):1387-98. doi: 10.3950/jibiinkoka.98.1387.
To examine the roles of alpha-adrenergic, beta-adrenergic and cholinergic agonists on the nasal secretion mechanism, the author repeated nasal methacholine (Mch, 1 time 20 mg) challenges ten times at 10-min intervals in 13 healthy male subjects. As it was difficult to obtain a sufficient amount of nasal secretion for analysis using simple nasal provocation tests with alpha-and beta-adrenergic agonists before repeated Mch challenges, alpha-adrenergic (phenylephrine, Phe, 2 mg) and beta-adrenergic (isoproterenol, Iso, 0.2 mg) agonists were administered into both nostrils. The effects of these agents on the volume of nasal secretions (Vol), the fucose volume (Fu, an index of glandular secretion) and the albumin volume (Alb, an index of vascular permeability) in the Mch induced nasal secretions were analyzed each time. Repeated Mch challenges revealed that the initial challenge produced the largest response as reflected by the Vol, Fu and Alb of the secretions, and by Vol and Fu production being almost constant during the 10 repetitions of Mch challenge at 10-min intervals. However, Alb demonstrated the greatest decrements. Phe administration before Mch challenges gave rise to Fu increasing more markedly while Alb was unchanged. On the other hand, that of Iso before, produced greater decreases in Vol, Fu and Alb levels. Therefore, it is suggested that repeated Mch challenge causes the maintenance of glandular secretion while stopping albumin permeability, that with pre-administration of alpha-agonists glandular secretion increases, and that pre-administration of beta-agonists inhibits glandular secretion. When both alpha- and beta-agonists are given before the challenge, however, there is no increase in vascular permeability.
为研究α-肾上腺素能、β-肾上腺素能和胆碱能激动剂在鼻分泌机制中的作用，作者对13名健康男性受试者每隔10分钟重复进行10次鼻内注射乙酰甲胆碱（Mch，每次20mg）激发试验。由于在重复Mch激发试验前，使用α-和β-肾上腺素能激动剂进行简单的鼻激发试验难以获得足够量的鼻分泌物用于分析，因此在双侧鼻孔中分别给予α-肾上腺素能激动剂（去氧肾上腺素，Phe，2mg）和β-肾上腺素能激动剂（异丙肾上腺素，Iso，0.2mg）。每次分析这些药物对Mch诱导的鼻分泌物中鼻分泌量（Vol）、岩藻糖量（Fu，腺分泌指标）和白蛋白量（Alb，血管通透性指标）的影响。重复Mch激发试验显示，首次激发产生的反应最大，这体现在分泌物的Vol、Fu和Alb上，并且在每隔10分钟重复进行10次Mch激发试验期间，Vol和Fu的产生几乎保持恒定。然而，Alb的下降最为明显。在Mch激发试验前给予Phe，可使Fu更明显地增加，而Alb无变化。另一方面，在Mch激发试验前给予Iso，则使Vol、Fu和Alb水平下降得更多。因此，提示重复Mch激发试验可使腺分泌维持，同时阻止白蛋白通透性增加；预先给予α-激动剂可增加腺分泌；预先给予β-激动剂可抑制腺分泌。然而，在激发试验前同时给予α-和β-激动剂时，血管通透性并无增加。
