Howard M D, Gordon D T, Pace L W, Garleb K A, Kerley M S
Department of Animal Science, University of Missouri, Columbia 65211, USA.
J Pediatr Gastroenterol Nutr. 1995 Oct;21(3):297-303. doi: 10.1097/00005176-199510000-00007.
Two experiments were conducted with neonatal pigs to determine the effects of feeding fructooligosaccharides on cecal and colonic microbiota, proliferation of cecal and colonic epithelial mucosa, and short-chain fatty acid concentrations in the cecum. Experiment 1 consisted of feeding neonatal pigs diets containing either 0 or 3 g fructooligosaccharies/L of formula for 15 days and then examining the large intestine for changes in cecal and proximal colonic microbiota; cecal pH; short-chain fatty acid concentrations; morphology of cecal, proximal, and distal colonic epithelial mucosa; gross necropsy; and histopathology. Supplementation with fructooligosacchariudes (FOS) did not alter cell counts of viable bifidobacterial organisms or total anaerobic microbiota, cecal pH, or concentrations of short-chain fatty acids. Cecal mucosal cell density and labeled cells increased with FOS consumption. Proximal colonic mucosal crypt height, leading edge, labeled cells, proliferation zone, and labeling index increased with FOS consumption. Distal colonic mucosal crypt height, leading edge, cell density, labeling index, and labeled cells increased with FOS consumption. Gross necropsy and histopathology found no significan lesions. In Experiment 2, neonatal pigs were fed diets containing either 0 or 3 g fructooligosaccharides/L of formula for 6 days. Fecal samples were collected on the first full day of feeding and on days 3 and 6 after initiation of feeding. On days 1 and 3, concentrations of bifidobacteria were similar between diets; however, on day 6, pigs consuming FOS tended to have greater numbers of bifidobacteria (p = 0.08). These data suggest dietary consumption of FOS will enhance bifidobacteria populations and prevent colonic epithelial mucosa atrophy in neonates fed an elemental diet.
进行了两项针对新生仔猪的实验，以确定喂食低聚果糖对盲肠和结肠微生物群、盲肠和结肠上皮黏膜增殖以及盲肠中短链脂肪酸浓度的影响。实验1包括给新生仔猪喂食含0或3克低聚果糖/升配方奶的日粮15天，然后检查大肠，观察盲肠和近端结肠微生物群的变化；盲肠pH值；短链脂肪酸浓度；盲肠、近端和远端结肠上皮黏膜的形态；大体尸检；以及组织病理学。补充低聚果糖（FOS）并未改变活双歧杆菌生物体或总厌氧微生物群的细胞计数、盲肠pH值或短链脂肪酸浓度。随着FOS摄入量的增加，盲肠黏膜细胞密度和标记细胞数量增加。随着FOS摄入量的增加，近端结肠黏膜隐窝高度、前沿、标记细胞、增殖区和标记指数增加。随着FOS摄入量的增加，远端结肠黏膜隐窝高度、前沿、细胞密度、标记指数和标记细胞数量增加。大体尸检和组织病理学检查未发现明显病变。在实验2中，给新生仔猪喂食含0或3克低聚果糖/升配方奶的日粮6天。在喂食的第一个完整日以及开始喂食后的第3天和第6天收集粪便样本。在第1天和第3天，不同日粮组的双歧杆菌浓度相似；然而，在第6天，摄入FOS的仔猪双歧杆菌数量往往更多（p = 0.08）。这些数据表明，在喂食要素饮食的新生儿中，日粮中摄入FOS将增加双歧杆菌数量并预防结肠上皮黏膜萎缩。
