人类免疫缺陷病毒1型（HIV-1）Gag衍生蛋白与5' HIV-1基因组RNA序列的特异性结合。
Specific binding of human immunodeficiency virus type 1 (HIV-1) Gag-derived proteins to a 5' HIV-1 genomic RNA sequence.
作者信息
Geigenmüller U, Linial M L
机构信息
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
出版信息
J Virol. 1996 Jan;70(1):667-71. doi: 10.1128/JVI.70.1.667-671.1996.
Abstract
We developed an in vitro binding assay to study the specific interaction between human immunodeficiency virus type 1 (HIV-1) RNA and the Gag polyprotein. Binding of the in vitro-expressed protein to in vitro-transcribed RNA was determined by altered migration of the protein in polyacrylamide gels. We found that a Gag precursor lacking the matrix domain bound specifically to HIV-1 RNA, while deletion of both matrix and capsid domains diminished the specificity of binding. Among several regions of HIV-1 RNA tested, strongest binding was seen with the 5'-most 261 nucleotides, while antisense RNA from the same region did not bind.
摘要
我们开发了一种体外结合试验，以研究1型人类免疫缺陷病毒（HIV-1）RNA与Gag多聚蛋白之间的特异性相互作用。通过聚丙烯酰胺凝胶中蛋白质迁移的改变来确定体外表达的蛋白质与体外转录的RNA的结合。我们发现，缺少基质结构域的Gag前体与HIV-1 RNA特异性结合，而基质和衣壳结构域的缺失则降低了结合的特异性。在测试的HIV-1 RNA的几个区域中，与最5'端的261个核苷酸结合最强，而来自同一区域的反义RNA不结合。
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