Borowsky R L, McClelland M, Cheng R, Welsh J
Department of Biology, New York University, Washington Square 10003, USA.
Mol Biol Evol. 1995 Nov;12(6):1022-32. doi: 10.1093/oxfordjournals.molbev.a040278.
Arbitrarily primed PCR (AP-PCR or RAPD) is a technique for producing species-specific DNA fingerprints. We tested the utility of AP-PCR as a source of phylogenetically informative characters in three separate experiments, using fishes of the genus Xiphophorus. We chose Xiphophorus as a standard of comparison, because evolutionary relationships within the genus have been studied repeatedly using a variety of techniques. We compared our results to a "classical" phylogenetic hypothesis synthesized from studies using morphological, pigmentation, and allozyme characters, and to a recent conflicting hypothesis constructed from DNA sequence data. The sequence-based hypothesis places the southern swordtail Xiphophorus clemenciae squarely within the platyfish, whereas the classical hypothesis separates the two groups. In addition, the two hypotheses differ in their clustering of species of northern swordtails. Our findings are in close accord with the classical hypothesis. Our results allow the strongest phylogenetic hypothesis yet for Xiphophorus and demonstrate the utility of AP-PCR for studying species relationships within vertebrate genera.
任意引物聚合酶链反应（AP-PCR或RAPD）是一种用于产生物种特异性DNA指纹图谱的技术。我们在三个独立的实验中，以剑尾鱼属鱼类为研究对象，测试了AP-PCR作为系统发育信息特征来源的实用性。我们选择剑尾鱼属作为比较标准，因为该属内的进化关系已通过多种技术进行了反复研究。我们将我们的结果与一个由形态学、色素沉着和等位酶特征研究综合而成的“经典”系统发育假说进行了比较，并与一个最近由DNA序列数据构建的相互冲突的假说进行了比较。基于序列的假说将南部剑尾鱼Xiphophorus clemenciae明确地置于剑尾鱼属内，而经典假说则将这两个群体分开。此外，这两个假说在北部剑尾鱼物种的聚类上也有所不同。我们的发现与经典假说高度一致。我们的结果为剑尾鱼属提供了迄今为止最有力的系统发育假说，并证明了AP-PCR在研究脊椎动物属内物种关系方面的实用性。
