由含γ-微管蛋白的环状复合体引发的微管组装成核作用。

Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.

作者信息

Zheng Y, Wong M L, Alberts B, Mitchison T

机构信息

Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448, USA.

出版信息

Nature. 1995 Dec 7;378(6557):578-83. doi: 10.1038/378578a0.

DOI:10.1038/378578a0

PMID:8524390

Abstract

The highly conserved protein gamma-tubulin is required for microtubule nucleation in vivo. When viewed in the electron microscope, a highly purified gamma-tubulin complex from Xenopus consisting of at least seven different proteins is seen to have an open ring structure. This complex acts as an active microtubule-nucleating unit which can cap the minus ends of microtubules in vitro.

摘要

高度保守的γ-微管蛋白在体内是微管成核所必需的。在电子显微镜下观察时，从非洲爪蟾中提取的高度纯化的γ-微管蛋白复合体（由至少七种不同蛋白质组成）呈现出开放的环状结构。该复合体作为一个活跃的微管成核单元，在体外能够封闭微管的负端。