Department of Genetics, University of Szeged, Szeged, Hungary.
Faculty of Science and Informatics, Doctoral School of Biology, University of Szeged, Szeged, Hungary.
Commun Biol. 2024 Oct 24;7(1):1385. doi: 10.1038/s42003-024-07090-9.
The initiation of microtubule formation is facilitated by γ-tubulin and γ-Tubulin Ring Complex (γ-TuRC) in various microtubule-organizing centers (MTOCs). While the heterogeneity of tissue-specific MTOCs and γ-TuRC in Drosophila testis has been described, their molecular composition and physiological significance are poorly understood. We investigated the testis-specific distribution and biochemical interaction of the canonical γ-TuRC proteins Grip163 and Grip84. We found that while Grip163 is present on the centrosome and basal body, Grip84 localizes to the centrosome and Golgi in spermatocytes and colocalizes with the testis-specific γ-Tubulin complexes (t-γ-TuC) at the basal body, apical nuclear tip, and near the elongated mitochondria after meiosis. We also showed the apical nuclear tip localization of some γ-TuRC interacting partners and proved their binding to t-γ-TuC proteins. These results highlight and prove the importance of the different γ-TuRCs in organizing the diverse MTOCs present during the extensive rearrangement of cell organelles during the spermatogenesis of Drosophila.
微管的形成起始于γ-微管蛋白和γ-微管蛋白环复合物（γ-TuRC）在各种微管组织中心（MTOC）中。虽然果蝇睾丸中组织特异性 MTOC 和 γ-TuRC 的异质性已被描述，但它们的分子组成和生理意义知之甚少。我们研究了经典的 γ-TuRC 蛋白 Grip163 和 Grip84 在睾丸中的特异性分布和生化相互作用。我们发现，虽然 Grip163 存在于中心体和基体上，但 Grip84 定位于精母细胞的中心体和高尔基体，并与睾丸特异性 γ-微管蛋白复合物（t-γ-TuC）在基体、核顶端和减数分裂后伸长的线粒体附近共定位。我们还显示了一些与 γ-TuRC 相互作用的伴侣在核顶端的定位，并证明了它们与 t-γ-TuC 蛋白的结合。这些结果突出并证明了不同的 γ-TuRC 在组织果蝇精子发生过程中细胞器官广泛重排期间存在的不同 MTOC 方面的重要性。
