Martín de las Mulas J, Gómez-Villamandos J C, Pérez J, Mozos E, Estrado M, Méndez A
Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Córdoba, Spain.
Res Vet Sci. 1995 Sep;59(2):124-7. doi: 10.1016/0034-5288(95)90045-4.
The immunohistochemical expression of the oncofetal proteins alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), and the intermediate filament proteins keratin and vimentin was analysed in 18 canine liver carcinomas. All the tumours other than hepatocellular carcinomas, with the exception of one poorly differentiated carcinoma, were AFP negative, and only cholangiocarcinomas and mixed (hepatocellular and cholangiocellular) carcinomas were CEA positive. All the histological types of tumours expressed high and low molecular weight keratins, and keratin and vimentin were both expressed in three tumours (one moderately differentiated hepatocellular carcinoma, one mixed carcinoma and one poorly differentiated carcinoma). The findings demonstrate the use of immunohistochemical staining methods for analysing the expression of some tumour markers in routinely processed tissue samples of canine liver carcinomas, and suggest that some of the tumour markers are correlated with histological types of tumour.
对18例犬肝癌组织中癌胚蛋白甲胎蛋白（AFP）和癌胚抗原（CEA）以及中间丝蛋白角蛋白和波形蛋白的免疫组化表达进行了分析。除肝细胞癌外，所有肿瘤（除1例低分化癌外）均为AFP阴性，只有胆管癌和混合性（肝细胞和胆管细胞）癌为CEA阳性。所有组织学类型的肿瘤均表达高分子量和低分子量角蛋白，3例肿瘤（1例中度分化肝细胞癌、1例混合癌和1例低分化癌）同时表达角蛋白和波形蛋白。这些发现证明了免疫组化染色方法可用于分析犬肝癌常规处理组织样本中某些肿瘤标志物的表达，并提示某些肿瘤标志物与肿瘤的组织学类型相关。
