华支睾吸虫病患者治疗前后血清中的抗体。

Antibodies in serum of patients with clonorchiasis before and after treatment.

作者信息

Lin Y L, Chen E R, Yen C M

机构信息

Department of Parasitology, Kaohsiung Medical College, Taiwan, ROC.

出版信息

Southeast Asian J Trop Med Public Health. 1995 Mar;26(1):114-9.

PMID:8525396

Abstract

Sera of 31 patients infected with Clonorchis sinensis were examined using fraction 1 antigen by ELISA during a one-year observation. The results of ELISA with Igs, IgG and IgA demonstrated high sensitivity (100%, 100% and 90%) and specificity (100%, 100% and 87%). Sera specific Igs and IgG were significantly decreased in the 3rd month after treatment with praziquantel (25mg/kg body weight in one dose), and IgA was significantly decreased in the 1st month (paired t-test, p < 0.05). No eggs were found in the stool after treatment. Detection of sera specific Igs, IgG and IgA by ELISA was combined with stool examination to evaluate the effect of praziquantel and the completeness of the cure.

摘要

在为期一年的观察期内，采用酶联免疫吸附测定（ELISA）法，使用1号抗原对31例华支睾吸虫感染患者的血清进行检测。IgM、IgG和IgA的ELISA检测结果显示出高敏感性（分别为100%、100%和90%）和高特异性（分别为100%、100%和87%）。用吡喹酮（25mg/kg体重，单次给药）治疗后第3个月，血清特异性IgM和IgG显著下降，而IgA在第1个月显著下降（配对t检验，p<0.05）。治疗后粪便中未发现虫卵。通过ELISA检测血清特异性IgM、IgG和IgA并结合粪便检查，以评估吡喹酮的疗效和治愈的彻底性。