Stöffler G, Fischer N, Rak K H, Ehrlich R
Mol Gen Genet. 1978 Oct 30;166(2):167-79. doi: 10.1007/BF00285919.
Antibodies were prepared in rabbits and sheep to rat liver ribosomes, ribosomal subunits, and to mixtures of proteins from the particles. The antisera were characterized by quantitative immunoprecipitation, by passive hemagglutination, by immunodiffusion on Ouchterlony plates, and by immunoelectrophoresis. While all the antisera contained antibodies specific for ribosomal proteins, none had precipitating antibodies against ribosomal RNA. Rat liver ribosomal proteins were more immunogenic in sheep than rabbits, and the large ribosomal subunit and its proteins were more immunogenic than those of the 40S subparticle. Antisera specific for one or the other ribosomal subunit could be prepared; thus it is unlikely that there are antigenic determinants common to the proteins of the two subunits. When ribosomes, ribosomal subunits, or mixtures of proteins were used as antigens the sera contained antibodies directed against a large number of the ribosomal proteins.
针对大鼠肝脏核糖体、核糖体亚基以及这些颗粒中的蛋白质混合物，在兔子和绵羊体内制备了抗体。通过定量免疫沉淀、被动血凝、在奥克特洛尼平板上进行免疫扩散以及免疫电泳对这些抗血清进行了表征。虽然所有抗血清都含有针对核糖体蛋白质的特异性抗体，但没有一种具有针对核糖体RNA的沉淀抗体。大鼠肝脏核糖体蛋白质在绵羊体内比在兔子体内更具免疫原性，并且大核糖体亚基及其蛋白质比40S亚颗粒的更具免疫原性。可以制备针对一种或另一种核糖体亚基的特异性抗血清；因此，两个亚基的蛋白质不太可能存在共同的抗原决定簇。当核糖体、核糖体亚基或蛋白质混合物用作抗原时，血清中含有针对大量核糖体蛋白质的抗体。
