Zuo X J, Jordan S C, Wilkinson A, Danovitch G M, Barba L, Schwieger J, Nast C C
Department of Pediatrics, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Transplantation. 1995 Dec 15;60(11):1360-2.
TH1 cytokines, including gamma-interferon (IFN), are critical in the initiation and progression of allograft rejection. As interleukin (IL)-12 up-regulates gamma-IFN, we assessed the role of IL-12 in human transplant rejection. Twenty renal allograft fine-needle aspirates from 19 patients were obtained, evaluated in the standard fashion, and assessed for gamma-IFN and IL-12p40 subunit mRNA levels using nested reverse transcriptase polymerase chain reaction. Ten aspirates demonstrated acute rejection by clinical criteria, and 9 of the 10 aspirates contained gamma-IFN while only 3 demonstrated IL-12; there were no distinguishing characteristics for these 3 patients with regard to therapy, or time of onset and severity of rejection. Seven patients without clinical or morphologic rejection failed to demonstrate gamma-IFN or IL-12. Three patients had discrepant findings; there was no morphologic rejection, yet all 3 patients contained gamma-IFN and 1 patient demonstrated rejection on subsequent biopsy. However, only 1 aspirate exhibited IL-12 and this patient had no documented subsequent rejection. This study confirms the association of gamma-IFN mRNA with acute rejection. In contrast, IL-12 mRNA does not appear to play a key role early in the rejection process.
包括γ干扰素（IFN）在内的TH1细胞因子在同种异体移植排斥反应的起始和进展中起关键作用。由于白细胞介素（IL）-12上调γ干扰素，我们评估了IL-12在人类移植排斥反应中的作用。从19例患者中获取了20份肾移植细针穿刺物，采用标准方式进行评估，并使用巢式逆转录聚合酶链反应评估γ干扰素和IL-12p40亚基mRNA水平。10份穿刺物根据临床标准显示为急性排斥反应，10份穿刺物中有9份含有γ干扰素，而只有3份显示有IL-12；这3例患者在治疗、排斥反应的发作时间和严重程度方面没有明显特征。7例无临床或形态学排斥反应的患者未显示γ干扰素或IL-12。3例患者有不一致的结果；没有形态学排斥反应，但所有3例患者都含有γ干扰素，1例患者在随后的活检中显示有排斥反应。然而，只有1份穿刺物显示有IL-12，且该患者随后没有记录到排斥反应。这项研究证实了γ干扰素mRNA与急性排斥反应之间的关联。相比之下，IL-12 mRNA在排斥反应过程早期似乎不发挥关键作用。
