Bruni R, Argentini C, D'Ugo E, Giuseppetti R, Ciccaglione A R, Rapicetta M
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Virology. 1995 Dec 1;214(1):229-34. doi: 10.1006/viro.1995.9936.
Frequent occurrence of woodchuck hepatitis virus DNA (WHV DNA) integration into or in proximity to myc oncogenes and in the win locus of cellular genome in woodchuck hepatocellular carcinomas (HCC) has been described by several authors. We report a further cellular locus as a recurrent target for WHV integration in woodchuck HCCs. A WHV DNA integration and its cellular flanking regions were cloned from a HCC developed in a chronically WHV-infected woodchuck. Sequence analysis showed integration of rearranged C, PreS1, and 5' truncated X regions of the WHV genome, located in a cellular locus previously described for WHV integration in another woodchuck HCC. The two integration sites are only about 0.5 kb apart. In addition to Alu-like repeats and a gag-like coding region, previously described, we found several features of MAR (matrix attachment region) chromosomal sequences in the normal cellular locus, leading us to predict that part of it might be a previously unrecognized MAR.
几位作者已描述了在土拨鼠肝细胞癌（HCC）中，土拨鼠肝炎病毒DNA（WHV DNA）频繁整合到myc癌基因内部或其附近以及细胞基因组的win基因座中。我们报告了土拨鼠HCC中作为WHV整合的一个反复出现靶点的另一个细胞基因座。从一只慢性感染WHV的土拨鼠所患的HCC中克隆出一个WHV DNA整合体及其细胞侧翼区域。序列分析显示，重排的WHV基因组C区、前S1区和5'端截短的X区整合到一个细胞基因座中，该基因座先前在另一只土拨鼠HCC中被描述为WHV整合位点。这两个整合位点相距仅约0.5 kb。除了先前描述的Alu样重复序列和gag样编码区外，我们在正常细胞基因座中发现了几个MAR（基质附着区域）染色体序列特征，这使我们预测其中一部分可能是一个先前未被识别的MAR。
