Moriuchi H, Moriuchi M, Cohen J I
Medical Virology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
Virology. 1995 Dec 1;214(1):256-8. doi: 10.1006/viro.1995.9932.
The varicella-zoster virus (VZV) immediate-early (IE) open reading frame 62 (ORF62) promoter sequence contains a CCAAT box, located between positions -117 and -113. Mutation of this motif resulted in a five-fold increase of promoter activity. In mobility shift assays, we demonstrated that NF-Y, a CCAAT box-binding protein critical for the expression of diverse eukaryotic genes, binds to this motif. Thus, the VZV IE ORF62 promoter contains a negative regulatory element that binds the ubiquitous transcriptional factor NF-Y.
水痘带状疱疹病毒（VZV）立即早期（IE）开放阅读框62（ORF62）启动子序列包含一个CCAAT框，位于-117至-113位之间。该基序的突变导致启动子活性增加了五倍。在凝胶迁移实验中，我们证明了NF-Y（一种对多种真核基因表达至关重要的CCAAT框结合蛋白）与该基序结合。因此，VZV IE ORF62启动子包含一个与普遍存在的转录因子NF-Y结合的负调控元件。
