Kalcklösch M, Kyriakopoulos A, Hammel C, Behne D
Hahn-Meitner-Institut Berlin, Department Trace Elements in Health and Nutrition, Germany.
Biochem Biophys Res Commun. 1995 Dec 5;217(1):162-70. doi: 10.1006/bbrc.1995.2759.
An inverse relationship between the Se status and the incidence of prostate cancer suggests a significant role of Se in this organ. After labeling of rats with 75Se and sodium dodecyl sulfate-polyacrylamide gel electrophoresis a strongly labeled prostatic 15-kDa protein band was found which was equally distributed among the different lobes. It was localized in the epithelial cells of isolated acini but did not appear in the prostatic secretion. By two-dimensional electrophoresis the band was resolved into three spots with pI-values around 4.5. The most strongly labeled spot stemmed from a cytosolic selenoprotein with an apparent native molecular mass of about 300 kDa which contained Se in the form of selenocysteine. The fact that with insufficient Se intake the element is preferentially incorporated into this compound as compared with glutathione peroxidase implies an important function of this newly found prostatic epithelial selenoprotein (PES).
硒状态与前列腺癌发病率之间的负相关关系表明硒在该器官中具有重要作用。用75硒标记大鼠并进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，发现一条强标记的前列腺15 kDa蛋白带，该带在不同叶中均匀分布。它定位于分离腺泡的上皮细胞中，但不出现在前列腺分泌物中。通过二维电泳，该带被解析为三个等电点值约为4.5的斑点。标记最强的斑点来自一种胞质硒蛋白，其表观天然分子量约为300 kDa，以硒代半胱氨酸的形式含有硒。与谷胱甘肽过氧化物酶相比，在硒摄入不足时该元素优先掺入该化合物这一事实意味着这种新发现的前列腺上皮硒蛋白（PES）具有重要功能。
