Belia S, Mannucci R, Lisciarelli M, Cacchio M, Fano G
Dipartimento di Biologia Cellulare e Molecolare, Università degli Studi di Perugia, Italy.
Cell Signal. 1995 May;7(4):389-95. doi: 10.1016/0898-6568(94)00092-p.
In PC12, a cellular line derived from a rat pheochromocytoma, ethanol (EtOH) induces a different effect depending on the concentration used. When resting cells are incubated with an alcohol concentration less than or equal to 120 mM, the [Ca2+]i increased with a double phase pattern. If the alcohol concentration was increased over 120-160 mM, EtOH reversed its effect and the [Ca2+]i decreased. This decrease was strongly inhibited if KCl-depolarized cells were used and was completely abolished if the substrate constituted EtOH-chronically treated cells. The Ca2+ increase is the consequence of an activation of L-type voltage-activated channels, while the other voltage-dependent channels (N-type), the receptor-operated channels and the Ca2+ extrusion pump present in these cells are not involved in EtOH action. These findings indicate that EtOH can induce (by different mechanisms) both potentiating and inhibiting effects on [Ca2+]i in PC12 cells in relation to the alcohol dose effectively present in the suspension medium.
在源自大鼠嗜铬细胞瘤的PC12细胞系中，乙醇（EtOH）根据所使用的浓度会产生不同的效应。当静止细胞与浓度小于或等于120 mM的酒精孵育时，细胞内钙离子浓度（[Ca2+]i）以双相模式增加。如果酒精浓度增加到超过120 - 160 mM，EtOH会逆转其效应，[Ca2+]i降低。如果使用氯化钾去极化的细胞，这种降低会受到强烈抑制；如果底物是经EtOH长期处理的细胞，则这种降低会完全消除。钙离子增加是L型电压激活通道激活的结果，而这些细胞中存在的其他电压依赖性通道（N型）、受体操纵通道和钙离子外排泵均不参与EtOH的作用。这些发现表明，相对于悬浮培养基中有效存在的酒精剂量，EtOH可通过不同机制对PC12细胞中的[Ca2+]i产生增强和抑制作用。
