Sun D, Woodland D L, Coleclough C, Wendling U, Reske K
Department of Immunology, St Jude Children's Research Hospital, Memphis, TN 38101, USA.
Int Immunol. 1995 Jul;7(7):1079-85. doi: 10.1093/intimm/7.7.1079.
We have analyzed the response of rat T cells to myelin basic protein (MBP) and the bacterial superantigen, staphylococcal enterotoxin E (SEE). Rat T cells reactive with MBP can respond to SEE presented by spleen cells but not to SEE presented by LOA, a rat T cell clone that expresses both I-A and I-E MHC class II molecules, even though LOA is much more efficient than splenic APC in the presentation of MBP. The inability of LOA to present superantigen is not due to a structural difference in MHC II molecules between LOA and the splenic APC or to differential expression of major accessory/adhesion molecules, including CD2, CD5, CD4 and CD44, on LOA. The non-responsiveness of SEE/LOA-induced T cells differs from anergy, in that such cells do not lose their subsequent responsiveness to either MBP or SEE. Our results demonstrate that: (i) MHC class II molecules (I-A and I-E) alone are insufficient for the activation of T cells by bacterial superantigen, (ii) failure to respond to antigen presented upon inappropriate APC or in inadequate doses may not necessarily represent anergy, and (iii) the quality of the T cell response towards certain ligands can be strongly influenced by the nature of the APC.
我们分析了大鼠T细胞对髓鞘碱性蛋白（MBP）和细菌超抗原葡萄球菌肠毒素E（SEE）的反应。与MBP反应的大鼠T细胞能够对脾细胞呈递的SEE作出反应，但对由同时表达I-A和I-E MHC II类分子的大鼠T细胞克隆LOA呈递的SEE无反应，尽管LOA在呈递MBP方面比脾抗原呈递细胞（APC）效率高得多。LOA不能呈递超抗原并非由于LOA与脾APC之间MHC II类分子的结构差异，也不是由于包括CD2、CD5、CD4和CD44在内的主要辅助/黏附分子在LOA上的差异表达。SEE/LOA诱导的T细胞无反应性不同于无反应状态，因为这类细胞对MBP或SEE都不会失去随后的反应性。我们的结果表明：（i）单独的MHC II类分子（I-A和I-E）不足以通过细菌超抗原激活T细胞；（ii）未能对不适当的APC呈递的抗原或剂量不足的抗原作出反应不一定代表无反应状态；（iii）T细胞对某些配体的反应质量可受到APC性质的强烈影响。
