Mulligan M S, Miyasaka M, Suzuki Y, Kawashima H, Iizuka M, Hasegawa A, Kiso M, Warner R L, Ward P A, Suzuki T
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA.
Int Immunol. 1995 Jul;7(7):1107-13. doi: 10.1093/intimm/7.7.1107.
Selectins promote adhesive interactions between leukocytes and activated endothelial cells, the adhesion being mediated by 'counter-receptors' on endothelial cells and consisting of oligosaccharide conjugates containing sialic acid and fucose. There are also suggestions that selectins bind sulfated compounds, including sulfatides. Intravenous infusion of selectin-reactive oligosaccharides has been found to prevent selectin-dependent inflammatory lung injury. In the current studies using two models of neutrophil and selectin-dependent acute lung injury in rats, sulfatide and its modified versions were infused i.v. and the protective effects determined. Naturally occurring sulfatide, synthetic sulfatides and sulfated ganglioside were highly protective against lung injury following systemic activation of complement. Desulfated sulfatide was inactive. The protective effects of synthetic sulfatides required sulfation of galactose in position 3. Sulfatide was also protective in the IgG immune complex model of lung injury. The protective effects of sulfatides were associated with reduced content of myeloperoxidase (derived from neutrophils) in lung tissue. These data indicate that sulfatides have significant in vivo protective effects in neutrophil and selectin-dependent models of lung injury.
选择素促进白细胞与活化内皮细胞之间的黏附相互作用，这种黏附由内皮细胞上的“反受体”介导，反受体由含有唾液酸和岩藻糖的寡糖缀合物组成。也有观点认为选择素可结合包括硫苷脂在内的硫酸化化合物。已发现静脉输注与选择素反应的寡糖可预防选择素依赖性炎性肺损伤。在当前使用大鼠中性粒细胞和选择素依赖性急性肺损伤两种模型的研究中，静脉注射硫苷脂及其修饰形式，并确定其保护作用。天然存在的硫苷脂、合成硫苷脂和硫酸化神经节苷脂对补体全身激活后的肺损伤具有高度保护作用。去硫酸化的硫苷脂无活性。合成硫苷脂的保护作用需要半乳糖3位硫酸化。硫苷脂在IgG免疫复合物肺损伤模型中也具有保护作用。硫苷脂的保护作用与肺组织中髓过氧化物酶（源自中性粒细胞）含量降低有关。这些数据表明，硫苷脂在中性粒细胞和选择素依赖性肺损伤模型中具有显著的体内保护作用。
