Hwang T L, Chen M F, Huang S F, Yu H C, Widjaja R, Yang J T, Liu M S, Lau Y T
Department of Surgery, Chang Gung College of Medicine and Technology, Chang Gung Memorial Hospital, Taipei, Taiwan ROC.
J Formos Med Assoc. 1995 Nov;94(11):655-9.
The muscarinic receptor changes in two subcellular fractions of rat myocardium during sepsis, the sarcolemma (SL) and light vesicles (LV), were studied. [3H]-quinuclidinyl benzilate ([3H]-QNB) was used as a radioligand. Sepsis was induced by cecal ligation and puncture (CLP). The septic rats had higher pulse rates and slightly higher blood glucose levels than control rats. The marker enzyme assays revealed that the SL fraction was enriched with 5'-nucleotidase and the Na(+)-K(+)-ATPase activity increased over 20-fold, while the LV fraction showed very little enrichment when compared with the homogenate. [3H]-QNB binding studies showed that Bmax increased by 58.8% in SL with no changes in LV during early sepsis (9 h post-CLP), but there was no significant change in the Kd value. These data indicate that muscarinic cholinergic receptors in rat heart SL increase during early sepsis. Since the muscarinic cholinergic receptors mediate parasympathetic modulation of myocardial contractility, changes in the number of muscarinic receptors in the cardiac SL may have a pathophysiologic significance in the development of hemodynamic changes during sepsis.
研究了脓毒症期间大鼠心肌两个亚细胞组分即肌膜（SL）和轻囊泡（LV）中的毒蕈碱受体变化。[3H] - 喹核醇基苯甲酸酯（[3H] - QNB）用作放射性配体。通过盲肠结扎和穿刺（CLP）诱导脓毒症。脓毒症大鼠的脉搏率高于对照大鼠，血糖水平略高。标志物酶分析显示，SL组分富含5'-核苷酸酶，Na(+)-K(+)-ATP酶活性增加超过20倍，而与匀浆相比，LV组分几乎没有富集。[3H] - QNB结合研究表明，在脓毒症早期（CLP后9小时），SL中的Bmax增加了58.8%，LV中无变化，但Kd值无显著变化。这些数据表明，脓毒症早期大鼠心脏SL中的毒蕈碱胆碱能受体增加。由于毒蕈碱胆碱能受体介导心肌收缩力的副交感神经调节，心脏SL中毒蕈碱受体数量的变化可能在脓毒症期间血流动力学变化的发生中具有病理生理学意义。
