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2,3-二脱氢-2,4-二脱氧-4-胍基-N-乙酰-D-神经氨酸（4-胍基-Neu5Ac2en）是甲型流感病毒和乙型流感病毒唾液酸酶的一种慢结合抑制剂。

2,3-didehydro-2,4-dideoxy-4-guanidino-N-acetyl-D-neuraminic acid (4-guanidino-Neu5Ac2en) is a slow-binding inhibitor of sialidase from both influenza A virus and influenza B virus.

作者信息

Hart G J, Bethell R C

机构信息

Department of Virology, Glaxo Research and Development Ltd., Glaxo Medicines Research Centre, Stevenage, Hertfordshire, U.K.

出版信息

Biochem Mol Biol Int. 1995 Jul;36(4):695-703.

PMID:8528132
Abstract

The effect of 2,3-didehydro-2,4-dideoxy-4-guanidino-N-acetyl-D-neuraminic acid (4-guanidino-Neu5Ac2en) on the sialidases from influenza virus reassortant X31 (which contains the sialidase from A/Aichi/2/68) and influenza virus B/Beijing/1/87 has been investigated. We find that 4-guanidino-Neu5Ac2en is a slow-binding inhibitor of both influenza A and influenza B virus sialidase, and that association and dissociation rate constants are almost identical for both enzymes. Furthermore, values for these rate constants are independent of whether purified enzyme or detergent-treated virus is used in the assays.

摘要

研究了2,3-二脱氢-2,4-二脱氧-4-胍基-N-乙酰-D-神经氨酸（4-胍基-Neu5Ac2en）对来自流感病毒重配株X31（含有来自A/爱知/2/68的唾液酸酶）和流感病毒B/北京/1/87的唾液酸酶的影响。我们发现4-胍基-Neu5Ac2en是甲型和乙型流感病毒唾液酸酶的慢结合抑制剂，并且两种酶的结合和解离速率常数几乎相同。此外，这些速率常数的值与测定中使用的是纯化酶还是经去污剂处理的病毒无关。

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