Mulimani V H
Department of Biochemistry, Gulbarga University, India.
Biochem Mol Biol Int. 1995 Jul;36(4):897-905.
The use of intracellular alpha-galactosidase from Gibberella fujikuroi to remove raffinose and stachyose in soymilk was studied. The optimum conditions for the enzymic hydrolysis of raffinose and stachyose was pH 5.5 to 6.0 at 55 degrees C. Alpha-galactosidase showed optimum activity at pH 5.0 and 50 degrees C with the substrate p-nitrophenyl-alpha-D-galacto-pyranoside (PNGP). The enzyme showed no detectable loss of activity when held more than 8 hr at 50 degrees C. Thin layer chromatography (TLC) revealed the following composition of oligosaccharides in local soybean variety: sucrose, 5.53%; raffinose, 1.95%; and stachyose, 6.1%. Investigation by TLC showed complete hydrolysis of raffinose and stachyose in 3 hr. HPLC analysis of hydrolyzate indicated complete hydrolysis of stachyose, and more than 60% hydrolysis of raffinose in 2.5 hr.
研究了利用来自藤仓赤霉菌的细胞内α-半乳糖苷酶去除豆浆中的棉子糖和水苏糖。棉子糖和水苏糖酶促水解的最佳条件是在55℃、pH 5.5至6.0。α-半乳糖苷酶以对硝基苯基-α-D-吡喃半乳糖苷（PNGP）为底物时，在pH 5.0和50℃显示出最佳活性。该酶在50℃保持8小时以上时，活性没有可检测到的损失。薄层色谱（TLC）显示当地大豆品种中低聚糖的组成如下：蔗糖5.53%；棉子糖1.95%；水苏糖6.1%。TLC研究表明，棉子糖和水苏糖在3小时内完全水解。水解产物的HPLC分析表明，水苏糖在2.5小时内完全水解，棉子糖水解超过60%。
