Aizawa S, Kamisaku H, Sado T
Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba, Japan.
Bone Marrow Transplant. 1995 Oct;16(4):603-9.
Our previous results in a murine model indicated that the GVL effect against radiation-induced leukemias could be induced in not only MHC-incompatible but also MHC-compatible allogeneic BMT, and that the intensity of the GVL effect induced in MHC-compatible allogeneic BMT varied among different leukemias and the donor/host strain combinations used. With the use of a radiation-induced T cell leukemia which followed the induction of the GVL effect in both MHC-compatible and -incompatible, allogeneic BMT, the role of T cell subsets in the development of the GVL effect and GVHD was studied. The results indicated that Lyt2+ T cells contaminating donor BM were consistently critical for the induction of the GVL effect in MHC-incompatible (B10) and -compatible (B10.BR and AKR) allogeneic BMT of leukemia-bearing C3H mice, but the depletion of L3T4+ T cells had no effect. In contrast, lethal GVHD induced by AKR donor lymph node cells was totally dependent on L3T4+ T cells, but the depletion of Lyt2+ T cells had no effect. On the other hand, both T cell subsets could cause lethal GVHD induced by MHC-incompatible (B10) and -compatible (B10.BR) allogeneic donors. The distinct roles of T cell subsets of AKR donors were confirmed by the preferential induction of the GVL effect with the AKR donor bone marrow mixed with lymph node cells which had been depleted of L3T4+ T cells.(ABSTRACT TRUNCATED AT 250 WORDS)
我们先前在小鼠模型中的研究结果表明，移植物抗白血病（GVL）效应不仅可在主要组织相容性复合体（MHC）不相容的同种异体骨髓移植（BMT）中诱导产生，也可在MHC相容的同种异体BMT中诱导产生，并且在MHC相容的同种异体BMT中诱导产生的GVL效应强度因白血病类型以及所用供体/宿主品系组合的不同而有所差异。利用一种辐射诱导的T细胞白血病模型，该模型在MHC相容和不相容的同种异体BMT中均可诱导产生GVL效应，我们研究了T细胞亚群在GVL效应和移植物抗宿主病（GVHD）发生过程中的作用。结果表明，污染供体骨髓的Lyt2⁺ T细胞对于在患有白血病的C3H小鼠的MHC不相容（B10）和相容（B10.BR和AKR）同种异体BMT中诱导GVL效应始终至关重要，但去除L3T4⁺ T细胞并无影响。相比之下，AKR供体淋巴结细胞诱导的致死性GVHD完全依赖于L3T4⁺ T细胞，但去除Lyt2⁺ T细胞并无影响。另一方面，这两种T细胞亚群均可导致由MHC不相容（B10）和相容（B10.BR）同种异体供体诱导的致死性GVHD。通过将AKR供体骨髓与已去除L3T4⁺ T细胞的淋巴结细胞混合优先诱导GVL效应，证实了AKR供体T细胞亚群的不同作用。（摘要截选至250词）
