Hayward I P, Bridle K R, Campbell G R, Underwood P A, Campbell J H
Department of Anatomical Sciences, University of Queensland, Australia.
Cell Biol Int. 1995 Oct;19(10):839-46. doi: 10.1006/cbir.1995.1019.
The effect on phenotypic expression of rabbit vascular smooth muscle cells (SMC) of the interstitial matrix proteins collagen I and fibronectin, the basal lamina proteins collagen IV and laminin, and the serum adhesion protein vitronectin was examined in culture. Experiments were performed in foetal calf serum stripped of fibronectin and vitronectin to eliminate their confounding effects. All the proteins promoted adhesion to the plastic culture dish (in a concentration dependent manner) of SMC freshly isolated from the artery wall. These cells had a high volume density of myofilaments (Vvmyo) in their cytoplasm. Laminin was best at maintaining SMC with a high Vvmyo (Vvmyo = 49.8%) followed by collagen IV (41.7%). Cells plated on vitronectin showed the lowest Vvmyo (31.3%). The results support the concept that the SMC basal lamina has a role in maintaining cells in the high Vvmyo phenotype.
在培养过程中，研究了间质基质蛋白I型胶原蛋白和纤连蛋白、基底层蛋白IV型胶原蛋白和层粘连蛋白以及血清粘附蛋白玻连蛋白对兔血管平滑肌细胞（SMC）表型表达的影响。实验在去除了纤连蛋白和玻连蛋白的胎牛血清中进行，以消除它们的干扰作用。所有这些蛋白质均促进了刚从动脉壁分离出的SMC对塑料培养皿的粘附（呈浓度依赖性）。这些细胞的细胞质中肌丝的体积密度（Vvmyo）很高。层粘连蛋白最有利于维持高Vvmyo的SMC（Vvmyo = 49.8%），其次是IV型胶原蛋白（41.7%）。接种在玻连蛋白上的细胞显示出最低的Vvmyo（31.3%）。结果支持这样一种观点，即SMC基底层在维持细胞的高Vvmyo表型中起作用。
