Tsai R T, Chang T C, Wang C R, Lee S L, Wang C J, Tsay G J
Department of Internal Medicine, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan, Republic of China.
Lupus. 1995 Aug;4(4):280-5. doi: 10.1177/096120339500400408.
Thyroid microsomal antibodies (Ms-Ab) are recently proved to be directed to thyroid peroxidase (TPO). The aim of this study was to investigate whether the sera of patients with systemic lupus erythematosus (SLE) contain anti-TPO antibodies (TPO-Ab) and whether these antibodies influence enzyme activity. Sera from patient with Hashimoto's thyroiditis was also studied. Serum samples were obtained from 37 patients with SLE, 20 patients with Hashimoto's thyroiditis and 20 healthy subjects. TPO-Ab were detected by immunoprecipitation using crude microsomal preparations or enzyme-linked immunoabsorbent assay (ELISA) with recombinant TPO. Positive TPO-Ab by ELISA were found in 11 (61%) of 18 patients with lupus whose serum contained Ms-Ab. Low levels of TPO-Ab also were found in three (16%) of 19 lupus sera that did not contain Ms-Ab. All patients with Hashimoto's thyroiditis had high levels of TPO-Ab in serum. When measured by ELISA, TPO-Ab were highly correlated with the results of a TPO immunoprecipitation assay and with the titers of Ms-Ab in patients with lupus (r = 0.83, n = 18, P < 0.01; r = 0.63, n = 18, P < 0.01) and in Hashimoto's thyroiditis (r = 0.89, n = 20, P < 0.01; r = 0.75, n = 20, P < 0.01). When evaluating the direct influence of TPO-Ab on the activity of TPO, we found no significant inhibition of enzymatic activity in both guaiacol and iodide assays by lupus sera in contrast with sera from Hashimoto's thyroiditis.(ABSTRACT TRUNCATED AT 250 WORDS)
甲状腺微粒体抗体（Ms-Ab）最近被证实是针对甲状腺过氧化物酶（TPO）的。本研究的目的是调查系统性红斑狼疮（SLE）患者的血清中是否含有抗TPO抗体（TPO-Ab）以及这些抗体是否影响酶活性。还对桥本甲状腺炎患者的血清进行了研究。从37例SLE患者、20例桥本甲状腺炎患者和20名健康受试者中获取血清样本。使用粗微粒体制剂通过免疫沉淀法或用重组TPO的酶联免疫吸附测定（ELISA）检测TPO-Ab。在18例血清中含有Ms-Ab的狼疮患者中，11例（61%）通过ELISA检测出TPO-Ab呈阳性。在19例不含Ms-Ab的狼疮血清中，也有3例（16%）检测到低水平的TPO-Ab。所有桥本甲状腺炎患者血清中的TPO-Ab水平都很高。通过ELISA测量时，TPO-Ab与TPO免疫沉淀测定结果以及狼疮患者（r = 0.83，n = 18，P < 0.01；r = 0.63，n = 18，P < 0.01）和桥本甲状腺炎患者（r = 0.89，n = 20，P < 0.01；r = 0.75，n = 20，P < 0.01）的Ms-Ab滴度高度相关。在评估TPO-Ab对TPO活性的直接影响时，我们发现与桥本甲状腺炎患者的血清相比，狼疮患者的血清在愈创木酚和碘化物测定中均未对酶活性产生显著抑制作用。（摘要截选至250字）
