Chen Z P, Bao Y D, Gorczyca W, Mohr G
Division of Neurosurgery, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis, Jewish General Hospital, McGill University, Montréal, Québec, Canada.
Artif Cells Blood Substit Immobil Biotechnol. 1995;23(5):597-604. doi: 10.3109/10731199509117974.
We have developed microcapsules using sodium alginate (SA) and Poly-L-Lysine (PLL). A factorial design method of screening was chosen to study influences of different experimental parameters on size and stability of the capsules. We found that air flow affects initial size of the capsules significantly, while the molecular weight (MW) of PLL and incubation time have a positive impact on the expansion when capsules are incubated in sodium citrate (SC). When the capsules were continuously shaken in an attempt to mimic in vivo environmental conditions, those capsules made with optimal parameters (0.1% PLL, 42,000 MW, incubated for 6 minutes; 1.5% SA, incubated for 4 minutes; SC bath 4 minutes; 25# needle; air flow 14L/min) were still intact after 30 days and not totally ruptured until 90 days, while those developed with less strict parameters were ruptured within 2 hours in 50%. We also encapsulated human pituitary adenoma cells using PLL of 80,000 MW and cultured them for 9 days. Adenoma cells, both encapsulated or non-encapsulated, secreted the same amount of hormones. Our preliminary study suggests that selecting optimal combinations of experimental parameters is essential in developing durable microcapsules, which may be potentially used for pituitary transplantation in vivo.
我们使用海藻酸钠（SA）和聚-L-赖氨酸（PLL）制备了微胶囊。选择析因设计筛选方法来研究不同实验参数对微胶囊大小和稳定性的影响。我们发现气流对微胶囊的初始大小有显著影响，而PLL的分子量（MW）和孵育时间在微胶囊于柠檬酸钠（SC）中孵育时对其膨胀有积极影响。当试图模拟体内环境条件对微胶囊进行持续振荡时，那些采用最佳参数制备的微胶囊（0.1% PLL，42000 MW，孵育6分钟；1.5% SA，孵育4分钟；SC浴4分钟；25号针头；气流14L/分钟）在30天后仍保持完整，直到90天才完全破裂，而那些制备参数要求没那么严格的微胶囊在50%的情况下会在2小时内破裂。我们还用80000 MW的PLL包裹了人垂体腺瘤细胞并培养了9天。无论是包裹的还是未包裹的腺瘤细胞，分泌的激素量相同。我们的初步研究表明，选择实验参数的最佳组合对于开发耐用的微胶囊至关重要，这些微胶囊可能潜在地用于体内垂体移植。
