Amores A, Bejar J, Alvarez M C
Departamento de Biologia Celular y Genética, Facultad de Ciencias, Universidad de Malaga, Spain.
Chromosome Res. 1995 Nov;3(7):423-6. doi: 10.1007/BF00713892.
High-resolution replication banding patterns have been obtained in prometaphase and metaphase chromosomes of the anguilliform fish species Echelus myrus by treating kidney cell cultures with 5-bromodeoxyuridine during the mid-late synthesis phase. The results show the superiority of the in vitro technique in obtaining a higher number of bands which permit an accurate identification of all chromosome pairs. Different replication patterns were compared with C-bands and silver-stained nucleolus organizer regions, providing information on the replication order of different chromatin regions.
通过在合成中后期用5-溴脱氧尿苷处理肾细胞培养物，在鳗形鱼类埃氏长吻鳗的前中期和中期染色体中获得了高分辨率的复制带型。结果表明，体外技术在获得更多条带方面具有优势，这些条带能够准确识别所有染色体对。将不同的复制模式与C带和银染核仁组织区进行了比较，提供了不同染色质区域复制顺序的信息。
