Dzienis-Koronkiewicz E, Debek W, Chyczewski L, Szyszko J, Worowski K
Department of Pediatric Surgery, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1995;40(1):138-47.
The total cathepsin D activities in the intestinal wall and in venous mesenteric and arterial systemic blood were investigated on the rats in untreated hemorrhagic shock lasting 60 minutes. We observed a decrease in cathepsin D activity in homogenates of respective segments of small and large bowels and an increase in the enzyme activity in blood serum of both origin. The shock resulted in lowering protein concentration in the intestinal wall and its increase in the mesenteric blood. We found a negative correlation between cathepsin D activity in the intestinal wall and its morphological destruction. Molecules of the enzyme, after liberation from lysosomes due to hemorrhagic shock, are translocated to the circulation and probably to the gut lumen. Liberation of the intestinal cathepsin D may contribute to the local damage and multiorgan failure in hemorrhagic shock.
对经历持续60分钟的未治疗失血性休克的大鼠，研究了肠壁、肠系膜静脉血和体循环动脉血中的组织蛋白酶D总活性。我们观察到小肠和大肠各段匀浆中组织蛋白酶D活性降低，而两种来源的血清中该酶活性增加。休克导致肠壁蛋白浓度降低，肠系膜血中蛋白浓度升高。我们发现肠壁中组织蛋白酶D活性与其形态学破坏之间呈负相关。由于失血性休克，该酶分子从溶酶体释放后转移至循环系统，可能还进入肠腔。肠组织蛋白酶D的释放可能导致失血性休克中的局部损伤和多器官功能衰竭。
