Pes D, Pelosi P
Istituto di Industrie Agrarie della Universita' degli Studi, Pisa, Italy.
Comp Biochem Physiol B Biochem Mol Biol. 1995 Nov;112(3):471-9. doi: 10.1016/0305-0491(95)00063-1.
After the isolation of two odorant-binding proteins (OBP-I and OBP-II) from mouse nasal tissue, we have purified two additional OBPs, which bind tritiated 2-isobutyl-3-methoxypyrazine. OBP-III is a homodimer with subunits of M(r) 22,000 and pI 4.2. OBP-IV is a homodimer with subunits of M(r) 21,000 and pI 4.85. N-terminal amino acid sequences indicate that OBP-III is identical in its first 40 amino acids to the mouse urinary protein, MUP-5, (ii) OBP-IV is > 90% identical in its first 30 amino acids to the MUP-4, OBP-II is nearly 80% similar in its first 40 amino acids to OBP-I of the rat, and both subunits of OBP-I are > 50% identical with hamster aphrodisin.
从小鼠鼻组织中分离出两种气味结合蛋白（OBP-I和OBP-II）后，我们又纯化出了另外两种能结合氚化2-异丁基-3-甲氧基吡嗪的OBP。OBP-III是一种同型二聚体，亚基的相对分子质量为22,000，等电点为4.2。OBP-IV是一种同型二聚体，亚基的相对分子质量为21,000，等电点为4.85。N端氨基酸序列表明：（i）OBP-III前40个氨基酸与小鼠尿蛋白MUP-5完全相同；（ii）OBP-IV前30个氨基酸与MUP-4的同源性超过90%；OBP-II前40个氨基酸与大鼠的OBP-I的相似性接近80%；OBP-I的两个亚基与仓鼠性诱素的同源性均超过50%。
