Schiffer D, Cavalla P, Di Sapio A, Giordana M T, Mauro A
II Department of Neurology, University of Turin, Italy.
Childs Nerv Syst. 1995 Sep;11(9):517-22. doi: 10.1007/BF00822841.
Thirty cases of hemispheric astrocytic tumors of childhood, consisting of 11 pilocytic astrocytomas, 2 fibrillary astrocytomas, 9 anaplastic astrocytomas, and 8 glioblastomas, were studied for the presence of p53 mutations and for immunohistochemical demonstrations of p53 and proliferation markers PCNA and Ki-67 MIB-1. The study was performed using polymerase chain reaction (PCR)-assisted single-strand conformation polymorphism analysis of exons 5-8 and direct sequence analysis of PCR products. For immunohistochemistry, DO1 and PAb 1801 were used. No mutation and no positivity for p53 protein were found in pilocytic astrocytomas. Mutations (at codons 144, 202, and 245) were found in 2 out of 8 glioblastomas and in 1 out of 9 anaplastic astrocytomas, whereas positive staining was found in 11 out of 17 malignant gliomas. Cases with mutations showed the highest p53 labeling index and also PCNA and MIB-1 labeling indices. The negative results in pilocytic astrocytomas are in line with the benign course of these tumors, whereas for malignant gliomas no difference seems to exist in comparison with adult cases.
对30例儿童半球星形细胞瘤进行了研究，其中包括11例毛细胞型星形细胞瘤、2例纤维型星形细胞瘤、9例间变性星形细胞瘤和8例胶质母细胞瘤，检测p53突变情况，并进行p53、增殖标志物PCNA和Ki-67 MIB-1的免疫组化检测。该研究采用聚合酶链反应（PCR）辅助的外显子5-8单链构象多态性分析及PCR产物直接测序分析。免疫组化采用DO1和PAb 1801。在毛细胞型星形细胞瘤中未发现p53突变及p53蛋白阳性。在8例胶质母细胞瘤中有2例、9例间变性星形细胞瘤中有1例发现突变（密码子144、202和245处），而在17例恶性胶质瘤中有11例呈阳性染色。有突变的病例显示出最高的p53标记指数以及PCNA和MIB-1标记指数。毛细胞型星形细胞瘤的阴性结果与这些肿瘤的良性病程一致，而对于恶性胶质瘤，与成人病例相比似乎没有差异。
