Matsumoto T, Fujii T, Yabe M, Oka K, Hoshi T, Sato K
First Department of Pathology, Juntendo University, School of Medicine, Tokyo, Japan.
Histopathology. 1998 Nov;33(5):446-52. doi: 10.1046/j.1365-2559.1998.00503.x.
To establish whether MIB-1 and p53 staining are useful for differentiating pilocytic astrocytomas and astrocytomas from anaplastic astrocytomas and glioblastomas. This study was restricted to children and young adults under 30 years of age because of the differences in p53 mutations between paediatric and adult astrocytomas.
Forty-five astrocytic tumours, including 18 pilocytic astrocytomas, 14 astrocytomas, four anaplastic astrocytomas and nine glioblastomas, from 45 children and young adults, between 1 and 29 years (mean 11 years) of age, were examined pathologically, and sections from paraffin-embedded blocks were used for MIB-1 and p53 immunostaining. The MIB-1 labelling index and the frequency and intensity of p53 staining in both the pilocytic astrocytoma and the astrocytoma group were significantly lower than in the anaplastic astrocytoma plus glioblastoma group (P < 0.001). In 11.1% (two of 18) of pilocytic astrocytomas and 42.9% (six of 14) of astrocytomas, immunoreactivity of either MIB-1 or p53 staining was of almost the same intensity as that of anaplastic astrocytomas and glioblastomas. However, in these cases, results using both MIB-1 and p53 stain differed from those for anaplastic astrocytomas and glioblastomas.
MIB-1 and p53 co-staining is very useful for differentiating pilocytic astrocytomas and astrocytomas from anaplastic astrocytomas and glioblastomas. However, MIB-1 or p53 staining alone cannot differentiate pilocytic astrocytomas and astrocytomas from anaplastic astrocytomas and glioblastomas.
确定MIB-1和p53染色是否有助于将毛细胞型星形细胞瘤和星形细胞瘤与间变性星形细胞瘤和胶质母细胞瘤区分开来。由于儿童和成人星形细胞瘤中p53突变存在差异，本研究仅限于30岁以下的儿童和年轻成人。
对45例年龄在1至29岁（平均11岁）的儿童和年轻成人的45例星形细胞肿瘤进行了病理检查，包括18例毛细胞型星形细胞瘤、14例星形细胞瘤、4例间变性星形细胞瘤和9例胶质母细胞瘤，取自石蜡包埋块的切片用于MIB-1和p53免疫染色。毛细胞型星形细胞瘤和星形细胞瘤组的MIB-1标记指数以及p53染色的频率和强度均显著低于间变性星形细胞瘤加胶质母细胞瘤组（P<0.001）。在11.1%（18例中的2例）的毛细胞型星形细胞瘤和42.9%（14例中的6例）的星形细胞瘤中，MIB-1或p53染色的免疫反应性强度与间变性星形细胞瘤和胶质母细胞瘤几乎相同。然而，在这些病例中，同时使用MIB-1和p53染色的结果与间变性星形细胞瘤和胶质母细胞瘤不同。
MIB-1和p53联合染色对于将毛细胞型星形细胞瘤和星形细胞瘤与间变性星形细胞瘤和胶质母细胞瘤区分开来非常有用。然而，单独的MIB-1或p53染色不能将毛细胞型星形细胞瘤和星形细胞瘤与间变性星形细胞瘤和胶质母细胞瘤区分开来。
