Chiari M, D'Alesio L, Consonni R, Righetti P G
Istituto di Chimica degli Ormoni, CNR, Milano, Italy.
Electrophoresis. 1995 Aug;16(8):1337-44. doi: 10.1002/elps.11501601221.
The average pore size value of gels containing polyacrylamide, covalently linked to agarose, was found to be 30% higher than the value of a regular N,N'-methylenebisacrylamide (Bis) cross-linked gel of the same %T. By increasing the agarose concentration (10% of the total amount of polyacrylamide), gels containing low amounts of acrylamide (1.5-2%) are reproducibly obtained; their pore sizes are 130% larger than the pore sizes of a 4%T, 3.3%C polyacrylamide gel. In general, mixed-bed matrices were found to be more elastic and mechanically stronger than classical polyacrylamide gels since an agarose-induced gelation process takes place during their polymerization.
含有与琼脂糖共价连接的聚丙烯酰胺的凝胶的平均孔径值，被发现比相同%T的常规N，N'-亚甲基双丙烯酰胺（双丙烯酰胺）交联凝胶的值高30%。通过增加琼脂糖浓度（占聚丙烯酰胺总量的10%），可重复性地获得含有少量丙烯酰胺（1.5 - 2%）的凝胶；它们的孔径比4%T、3.3%C聚丙烯酰胺凝胶的孔径大130%。一般来说，混合床基质被发现比传统聚丙烯酰胺凝胶更具弹性且机械强度更高，因为在其聚合过程中会发生琼脂糖诱导的凝胶化过程。
