Hermitte L, Atlan-Gepner C, Payan M J, Mehelleb M, Vialettes B
Service de Nutrition, Endocrinologie, Maladies métaboliques, Hôpital Sainte-Marguerite, Marseille, France.
Diabete Metab. 1995 Oct;21(4):261-8.
Pregestimil, a hypoallergenic infant formula in which casein hydrolysate replaces protein, protects NOD mice against diabetes, a T-cell-mediated autoimmune disease. Female and cyclosphosphamide (Cy)-treated male NOD mice were used to assess whether a modification of cellular immune mechanisms occurred when animals were fed Pregestimil from weaning to 110 days of life. Insulitis, sialitis and thyroiditis were observed, and the splenic T-cell proliferative response was measured. The ability of splenic T-cells of NOD mice in the Pregestimil group to transfer diabetes adoptively to young irradiated male NOD mice was also assessed. Pregestimil protected female NOD mice against spontaneous diabetes and male NOD mice against acute Cy-induced diabetes. Addition of bovine serum albumin (10%) to the diet did not alter the preventive effect. The Pregestimil diet also lessened insulitis severity in Cy-treated males, though not in females. Sialitis and thyroiditis, observed mainly in females, were not modified by the diets. The TCR-mediated proliferative response of splenocytes tended to increase specifically in Pregestimil-fed and Cy-treated males. Sensitivity to IL-2 was improved. In females, the TCR-mediated proliferative response and the ability of T cells to transfer diabetes adoptively were unchanged. It is concluded that the protective effect of Pregestimil against diabetes in NOD mice cannot be explained by major changes in peripheral immune response.
普瑞米尔（Pregestimil）是一种低敏婴儿配方奶粉，其中酪蛋白水解物替代了蛋白质，可保护非肥胖糖尿病（NOD）小鼠免受糖尿病（一种T细胞介导的自身免疫性疾病）的侵害。使用雌性和经环磷酰胺（Cy）处理的雄性NOD小鼠来评估动物从断奶至110日龄期间喂食普瑞米尔时细胞免疫机制是否发生改变。观察到胰岛炎、涎腺炎和甲状腺炎，并测量了脾脏T细胞增殖反应。还评估了普瑞米尔组NOD小鼠脾脏T细胞将糖尿病过继转移给年轻受照射雄性NOD小鼠的能力。普瑞米尔可保护雌性NOD小鼠免受自发性糖尿病的侵害，并保护雄性NOD小鼠免受急性Cy诱导的糖尿病的侵害。在饮食中添加10%的牛血清白蛋白不会改变预防效果。普瑞米尔饮食也减轻了Cy处理的雄性小鼠的胰岛炎严重程度，但对雌性小鼠没有作用。涎腺炎和甲状腺炎主要在雌性小鼠中观察到，饮食对其没有影响。在喂食普瑞米尔和经Cy处理的雄性小鼠中，脾细胞的TCR介导的增殖反应倾向于特异性增加。对白细胞介素-2的敏感性提高。在雌性小鼠中，TCR介导的增殖反应和T细胞过继转移糖尿病的能力没有变化。得出的结论是，普瑞米尔对NOD小鼠糖尿病的保护作用不能通过外周免疫反应的重大变化来解释。
