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Escherichia coli expression vectors containing a protein kinase recognition motif, His6-tag and hemagglutinin epitope.

作者信息

Kelman Z, Yao N, O'Donnell M

机构信息

Microbiology Department Hearst Research Foundation, Cornell University Medical College, New York, NY 10021, USA.

出版信息

Gene. 1995 Dec 1;166(1):177-8. doi: 10.1016/0378-1119(95)00556-7.

DOI:10.1016/0378-1119(95)00556-7
PMID:8529886
Abstract

Escherichia coli expression vectors, based on the pET system, were constructed to allow fusion of a protein kinase (PK) recognition motif, a hemagglutinin (HG) epitope-tag and a His6-tag at the N-terminal portion of a protein of interest. The fusion proteins, that result from expression using these vectors, can be phosphorylated in vitro using cAMP-dependent PK, immunoprecipitated using monoclonal antibody against the HG-epitope, and can be rapidly purified using a Ni2+ column.

摘要

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