嗜氢假单胞菌膜结合氢化酶编码基因（hupS和hupL）的克隆与测序

Cloning and sequencing of the membrane-bound hydrogenase-encoding genes (hupS and hupL) from Pseudomonas hydrogenovora.

作者信息

Ohtsuki T, Okazaki T, Endo Y, Kita Y, Shimosaka M, Okazaki M

机构信息

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.

出版信息

Gene. 1995 Dec 1;166(1):89-93. doi: 10.1016/0378-1119(95)00580-4.

DOI:10.1016/0378-1119(95)00580-4

PMID:8529899

Abstract

The membrane-bound hydrogenase (Hdg)-encoding structural genes were isolated from the hydrogen-utilizing bacterium Pseudomonas hydrogenovora (Ph). Nucleotide sequence analysis revealed two genes, hupS and hupL. The hupS gene encoded a 363-amino-acid (aa) polypeptide (40.4 kDa). The deduced aa sequence contained a putative 43-aa leader peptide sequence. The hupL gene started at 55 bp downstream from the stop codon of hupS and encoded a 622-aa polypeptide (69.3 kDa). The disruption of Ph hupS resulted in the loss of Hdg activity.

摘要

从利用氢气的细菌食氢假单胞菌（Ph）中分离出编码膜结合氢化酶（Hdg）的结构基因。核苷酸序列分析揭示了两个基因，hupS和hupL。hupS基因编码一个363个氨基酸（aa）的多肽（40.4 kDa）。推导的氨基酸序列包含一个推定的43个氨基酸的前导肽序列。hupL基因从hupS终止密码子下游55 bp处开始，编码一个622个氨基酸的多肽（69.3 kDa）。Ph hupS的破坏导致Hdg活性丧失。