Hamasaki K, Doi Y, Sakamoto Y, Kashimura M, Fujimoto S
Department of Obstetrics and Gynecology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu.
Fukuoka Igaku Zasshi. 1995 Oct;86(10):389-97.
The remarkable contraction of small arteries occurred in the perimetrium of the uteri with leiomyomas by a presurgical medication P6th the luteinizing hormone-releasing hormone (LHRH) agonist (buserelin). The contracted vessels showed a significant increase in number of the Weibel-Palade (WP) bodies which occasionally underwent the degranulation and the discharge in a manner of exocytosis. By immunoelectron microscopy, localizations of endothelin (ET)-1 immunoreactive gold particles on the WP bodies as well as on cisterns of the rough endoplasmic reticulum (rER) and Golgi apparatus were pronounced in the buserelin-medicated group when compared to the non-medicated one. These findings indicate that the increase of the WP bodies storing ET-1, of which synthesis in the rER-Golgi system enhances, predominantly occurred in the small arteries in the perimetrium by the buserelin-medication prior to the hysterectomy. ET-1, released into the subendothelial layer, seems to play an important role in the contraction of the vascular media in a manner of paracrine.
术前使用促黄体生成激素释放激素（LHRH）激动剂（布舍瑞林）P6后，子宫肌瘤子宫肌层周围的小动脉出现明显收缩。收缩的血管中，魏贝尔-帕拉德小体（WP小体）数量显著增加，这些小体偶尔会以胞吐方式发生脱颗粒和释放。免疫电子显微镜检查显示，与未用药组相比，布舍瑞林用药组中，WP小体以及粗面内质网（rER）和高尔基体池上内皮素（ET）-1免疫反应性金颗粒的定位更为明显。这些发现表明，储存ET-1的WP小体增加，且其在rER-高尔基体系统中的合成增强，这主要发生在子宫切除术前布舍瑞林用药所致的子宫肌层小动脉中。释放到内皮下层的ET-1似乎以旁分泌方式在血管中层收缩中起重要作用。
