Bonner C A, Loftus S K, Wasmuth J J
Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717, USA.
Genomics. 1995 Jul 20;28(2):206-11. doi: 10.1006/geno.1995.1132.
The human CDX1 gene has been isolated from a small intestine cDNA library using a murine Cdx1 cDNA probe. The nucleotide sequence of CDX1 is 81% identical to murine Cdx1 and predicts a 265-amino-acid protein with 85% identity to the mouse protein (98% identity, including conservative amino acid changes). The CDX1 locus has been mapped to a cosmid contig from chromosome 5q31-q33, placing CDX1 approximately 100 kb distal to CSFIR. Expression of CDX1 in adults appears to be limited to the intestine and colon by Northern analysis, suggesting a possible role in the terminal differentiation of the intestine. Further analysis of CDX1 should elucidate the function of caudal-type homeobox genes in human development.
利用小鼠Cdx1 cDNA探针从小肠cDNA文库中分离出人类CDX1基因。CDX1的核苷酸序列与小鼠Cdx1有81%的同源性，并预测出一种由265个氨基酸组成的蛋白质，该蛋白质与小鼠蛋白质的同源性为85%（包括保守氨基酸变化在内的同源性为98%）。CDX1基因座已被定位到5号染色体q31 - q33区域的一个黏粒重叠群上，使CDX1位于CSFIR远端约100 kb处。通过Northern分析，CDX1在成体中的表达似乎仅限于小肠和结肠，提示其在肠道终末分化中可能发挥作用。对CDX1的进一步分析应能阐明尾型同源框基因在人类发育中的功能。
