Blair H J, Ho M, Monaco A P, Fisher S, Craig I W, Boyd Y
Genetics Division, MRC Radiobiology Unit, Chilton, Oxon, United Kingdom.
Genomics. 1995 Jul 20;28(2):305-10. doi: 10.1006/geno.1995.1146.
The murine homologues of the loci for McLeod syndrome (XK), Dent's disease (CICN5), and synaptophysin (SYP) have been mapped to the proximal region of the mouse X chromosome and positioned with respect to other conserved loci in this region using a total of 948 progeny from two separate Mus musculus x Mus spretus backcrosses. In the mouse, the order of loci and evolutionary breakpoints (EB) has been established as centromere-(DXWas70, DXHXF34h)-EB-Clcn5-(Syp, DXMit55, DXMit26)-Tfe3-Gata1-EB-Xk-Cybb-telomere. In the proximal region of the human X chromosome short arm, the position of evolutionary breakpoints with respect to key loci has been established as DMD-EB-XK-PFC-EB-GATA1-C1CN5-EB-DXS1272E-ALAS2-E B-DXF34-centromere. These data have enabled us to construct a high-resolution genetic map for the approximately 3-cM interval between DXWas70 and Cybb on the mouse X chromosome, which encompasses 10 loci. This detailed map demonstrates the power of high-resolution genetic mapping in the mouse as a means of determining locus order in a small chromosomal region and of providing an accurate framework for the construction of physical maps.
已将麦克劳德综合征（XK）、丹特病（CICN5）和突触素（SYP）基因座的小鼠同源基因定位到小鼠X染色体的近端区域，并利用来自两个独立的小家鼠× 斯氏小家鼠回交的总共948个后代，相对于该区域的其他保守基因座确定了它们的位置。在小鼠中，基因座顺序和进化断点（EB）已确定为着丝粒-（DXWas70，DXHXF34h）-EB-Clcn5-（Syp，DXMit55，DXMit26）-Tfe3-Gata1-EB-Xk-Cybb-端粒。在人类X染色体短臂的近端区域，进化断点相对于关键基因座的位置已确定为DMD-EB-XK-PFC-EB-GATA1-C1CN5-EB-DXS1272E-ALAS2-EB-DXF34-着丝粒。这些数据使我们能够构建小鼠X染色体上DXWas70和Cybb之间约3厘摩区间的高分辨率遗传图谱，该区间包含10个基因座。这一详细图谱证明了小鼠中高分辨率遗传图谱作为确定小染色体区域基因座顺序以及为构建物理图谱提供准确框架的手段的强大作用。
