Sutton K A, Wilkinson M F
Department of Immunology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA.
Genomics. 1997 Oct 15;45(2):447-50. doi: 10.1006/geno.1997.4946.
The Pem gene encodes a homeodomain-containing protein expressed in reproductive tissue that may function as a transcription factor regulating spermatogenesis and sperm maturation. We have mapped the Pem gene to the proximal end of the mouse X chromosome, placing it within the Hprt region. Based on the mapping of Pem and other loci in three separate Mus musculus x Mus spretus backcross panels, we established the order of markers within this segment of the Hprt region as: Agtr2-Pem-Ant2-DXMit50-Lamp2-DXMit49. In contrast to some other regions of the X chromosome, which have been rearranged during the evolution of mammals, we show that the order of gene loci within this Hprt region is conserved in mice and human. The finding that the mouse Ant2 and Pem loci are tightly linked suggests that human ANT2 may be useful as a marker for isolating the human PEM gene, which has been impervious to cloning by conventional hybridization methods because of its rapid evolution.
Pem基因编码一种在生殖组织中表达的含同源结构域的蛋白质，该蛋白质可能作为一种转录因子，调节精子发生和精子成熟。我们已将Pem基因定位到小鼠X染色体的近端，使其位于次黄嘌呤磷酸核糖转移酶（Hprt）区域内。基于在三个独立的小家鼠× 西班牙小鼠回交群体中对Pem和其他基因座的定位，我们确定了Hprt区域这一片段内标记的顺序为：Agtr2-Pem-Ant2-DXMit50-Lamp2-DXMit49。与X染色体在哺乳动物进化过程中发生重排的其他一些区域不同，我们发现该Hprt区域内基因座的顺序在小鼠和人类中是保守的。小鼠Ant2和Pem基因座紧密连锁这一发现表明，人类ANT2可能作为分离人类PEM基因的一个标记，由于其快速进化，传统杂交方法一直无法克隆该基因。
