Yoshioka H, Inoguchi K, Khaleduzzaman M, Ninomiya Y, Andrikopoulos K, Ramirez F
Department of Molecular Biology and Biochemistry, Okayama University Medical School, Japan.
Genomics. 1995 Jul 20;28(2):337-40. doi: 10.1006/geno.1995.1151.
Several overlapping cDNA clones corresponding to the entire coding sequence of the mouse alpha 1(XI) collagen gene (Col11 a1) were isolated. The conceptual amino acid translation indicated a high degree of sequence identity (93%) with the human alpha 1(XI) chain. The cloning experiments also revealed alternative splicing of the sequence coding for 85 residues located within the acidic region of the amino-globular domain of alpha 1(XI). Analysis of RNA samples from different embryonic tissues suggested that alternative splicing might be confined to tissue destined to become bone.
分离出了几个与小鼠α1（XI）胶原蛋白基因（Col11a1）的整个编码序列相对应的重叠cDNA克隆。概念性氨基酸翻译表明与人类α1（XI）链具有高度的序列同一性（93%）。克隆实验还揭示了α1（XI）氨基球状结构域酸性区域内编码85个残基的序列存在可变剪接。对来自不同胚胎组织的RNA样本的分析表明，可变剪接可能局限于注定会形成骨骼的组织。
