Wong L J, O'Brien W E
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Genomics. 1995 Jul 20;28(2):341-3. doi: 10.1006/geno.1995.1152.
Adenylosuccinate lyase catalyzes two similar reactions in the de novo purine biosynthetic pathway; the cleavage of succinylaminoimidazole carboxamide ribotide to aminoimidazole carboxamide ribotide and fumarate and the cleavage of adenylosuccinate to adenylate and fumarate. Adenylosuccinate lyase is also a participant in the purine nucleotide cycle which plays an important role in maintaining the AMP levels in muscle. In order to understand the structure/function and evolutionary relationships of the members of the fumarate gene family and to evaluate the possible existence of tissue specific isoforms of adenylosuccinate lyase, we have isolated and characterized the murine cDNA and gene encoding adenylosuccinate lyase. The cDNA has 94% and 87% identity to the human sequence at the amino acid and nucleotide levels respectively. The gene is about 27 kb and contains 13 exons. Comparison of the exon/intron structure of this gene with the argininosuccinate lyase gene did not suggest gene duplication or exon shuffling as a mechanism of evolution in the fumarate gene family.
腺苷酸琥珀酸裂解酶在嘌呤从头生物合成途径中催化两个相似的反应；琥珀酰氨基咪唑甲酰胺核糖核苷酸裂解为氨基咪唑甲酰胺核糖核苷酸和富马酸，以及腺苷酸琥珀酸裂解为腺苷酸和富马酸。腺苷酸琥珀酸裂解酶也是嘌呤核苷酸循环的参与者，该循环在维持肌肉中的AMP水平方面起重要作用。为了了解富马酸基因家族成员的结构/功能和进化关系，并评估腺苷酸琥珀酸裂解酶组织特异性同工型的可能存在，我们分离并鉴定了编码腺苷酸琥珀酸裂解酶的小鼠cDNA和基因。该cDNA在氨基酸和核苷酸水平上与人序列的同一性分别为94%和87%。该基因约27kb，包含13个外显子。将该基因的外显子/内含子结构与精氨琥珀酸裂解酶基因进行比较，并未表明基因重复或外显子重排是富马酸基因家族进化的机制。
