Lidmer A S, Kannius M, Lundberg L, Bjursell G, Nilsson J
Department of Molecular Biology, Göteborg University, Medicinaregatan 9C, S-413 90 Göteborg, Sweden; Astra Hässle AB, Mölndal, Sweden.
Genomics. 1995 Sep 1;29(1):115-22. doi: 10.1006/geno.1995.1221.
DNA hybridization was used to isolate a 2.04-kb cDNA encoding carboxyl ester lipase (CEL) from a mouse lactating mammary gland, lambda gt10 cDNA library. The cDNA sequence translated into a protein of 599 amino acids, including 20 amino acids of a putative signal peptide. Comparison of the deduced amino acid sequence of the mouse CEL with CEL from five other species revealed that there is a high degree of homology between the different species. The mouse CEL gene was also isolated and found to span approximately 7.2 kb and to include 11 exons. This organization is similar to those of the recently reported human and rat CEL genes. We have also analyzed expression of the CEL gene in the mammary glands from other species by performing a Northern blot analysis with RNA from goat and cow. The results show that the gene is expressed in both species.
利用DNA杂交技术从小鼠泌乳乳腺λgt10 cDNA文库中分离出一个编码羧基酯酶（CEL）的2.04 kb cDNA。该cDNA序列翻译为一个含599个氨基酸的蛋白质，其中包括一段由20个氨基酸组成的假定信号肽。将推导的小鼠CEL氨基酸序列与其他五个物种的CEL进行比较，结果显示不同物种之间存在高度同源性。还分离出了小鼠CEL基因，发现其跨度约为7.2 kb，包含11个外显子。这种结构与最近报道的人类和大鼠CEL基因相似。我们还通过用山羊和奶牛的RNA进行Northern印迹分析，研究了其他物种乳腺中CEL基因的表达情况。结果表明该基因在这两个物种中均有表达。
