Petrini J H, Walsh M E, DiMare C, Chen X N, Korenberg J R, Weaver D T
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Genomics. 1995 Sep 1;29(1):80-6. doi: 10.1006/geno.1995.1217.
Mutation of the Saccharomyces cerevisiae RAD52 epistasis group gene, MRE11, blocks meiotic recombination, confers profound sensitivity to double-strand break damage, and has a hyperrecombinational phenotype in mitotic cells. We isolated a highly conserved human MRE11 homologue using a two-hybrid screen for DNA ligase I-interacting proteins. Human MRE11 shares approximately 50% identity with its yeast counterpart over the N-terminal half of the protein. MRE11 is expressed at the highest levels in proliferating tissues, but is also observed in other tissues. The MRE11 locus maps to human chromosome 11q21 in a region frequently associated with cancer-related chromosomal abnormalities. A MRE11-related locus was found on chromosome 7q11.2-q11.3.
酿酒酵母RAD52上位性群基因MRE11发生突变会阻断减数分裂重组，使细胞对双链断裂损伤产生高度敏感性，并且在有丝分裂细胞中具有高重组表型。我们通过双杂交筛选与DNA连接酶I相互作用的蛋白，分离出了一个高度保守的人类MRE11同源物。人类MRE11在蛋白质的N端一半区域与其酵母对应物具有约50%的同一性。MRE11在增殖组织中表达水平最高，但在其他组织中也可观察到。MRE11基因座定位于人类染色体11q21上一个经常与癌症相关染色体异常相关的区域。在染色体7q11.2 - q11.3上发现了一个与MRE11相关的基因座。
