酶联免疫吸附测定法（ELISA）与高效液相色谱法（HPLC）用于测定主动脉组织弹性蛋白中锁链素或异锁链素的比较。

Comparison of ELISA and HPLC for the determination of desmosine or isodesmosine in aortic tissue elastin.

作者信息

Osakabe T, Seyama Y, Yamashita S

机构信息

Drug Discovery Laboratory, Hoechst Japan Limited, Saitama, Japan.

出版信息

J Clin Lab Anal. 1995;9(5):293-6. doi: 10.1002/jcla.1860090503.

DOI:10.1002/jcla.1860090503

PMID:8531009

Abstract

We developed a rapid and simple method for estimating tissue elastin content by measuring desmosine (D) in tissue hydrolysates by competitive ELISA. We compared the ELISA previously reported HPLC methods. When D or isodesmosine (ID) in hydrolysate of the same elastin preparation were measured by the two different methods, a good linear relationship was obtained (r = 0.854 for human aorta or r = 0.938 for rabbit aorta, respectively). The ELISA method can detect as little as 6 pmol/ml and it may be useful in monitoring elastin metabolism in patients with various connective tissue diseases.

摘要

我们开发了一种快速简便的方法，通过竞争性酶联免疫吸附测定法（ELISA）测量组织水解产物中的锁链素（D）来估计组织弹性蛋白含量。我们将该ELISA方法与先前报道的高效液相色谱法（HPLC）进行了比较。当用两种不同方法测量同一弹性蛋白制剂水解产物中的D或异锁链素（ID）时，获得了良好的线性关系（人主动脉的r = 0.854，兔主动脉的r = 0.938）。ELISA方法的检测下限低至6 pmol/ml，可能有助于监测各种结缔组织疾病患者的弹性蛋白代谢。