Kadam A L, Fateh M, Naz R K
New York Fertility Institute, NY, USA.
J Reprod Immunol. 1995 May;29(1):19-30. doi: 10.1016/0165-0378(95)00928-e.
The effects of purified human sperm fertilization antigen-1 (FA-1), affinity-purified monoclonal Fab' antibody to FA-1, and monoclonal Fab' antibody to phosphotyrosine residues on human sperm-zona interaction were investigated. The purified FA-1 antigen completely blocked sperm binding to zona pellucida (P < 0.0001). Also, the monoclonal Fab' antibodies to FA-1 antigen and phosphotyrosine residues significantly (P < 0.05) reduced sperm-zona pellucidae and the antibodies were preincubated with sperm before insemination and not vice versa. These results suggest that the tyrosine phosphorylation especially of FA-1 antigen has an important role in zona pellucida receptor recognition and binding. These findings also suggest that FA-1 antigen may be the sperm receptor involved in zona pellucida binding in humans.
研究了纯化的人精子受精抗原-1（FA-1）、针对FA-1的亲和纯化单克隆Fab'抗体以及针对磷酸酪氨酸残基的单克隆Fab'抗体对人精子-透明带相互作用的影响。纯化的FA-1抗原完全阻断精子与透明带的结合（P < 0.0001）。此外，针对FA-1抗原和磷酸酪氨酸残基的单克隆Fab'抗体显著（P < 0.05）减少精子与透明带的结合，并且抗体在授精前与精子预孵育，而不是相反。这些结果表明，特别是FA-1抗原的酪氨酸磷酸化在透明带受体识别和结合中起重要作用。这些发现还表明，FA-1抗原可能是人类中参与透明带结合的精子受体。
