Suzuki A, Aizawa S, Iwase O, Kuriyama K, Nagasu M, Kawanishi Y, Yaguchi M, Nehashi Y, Nakano M, Yoshikawa O
First Department of Internal Medicine, Tokyo Medical College.
Rinsho Ketsueki. 1995 Oct;36(10):1193-8.
A 54-year-old man was admitted with fatigue. The peripheral blood count showed leukocytosis (9, 600/microliters), including 76% granular lymphocytes (GLs), which expressed CD2, 3, 8, 16 and HLA-DR, and anemia (hemoglobin 8.1 g/dl). He was diagnosed as having T cell type-granular lymphocyte proliferative disorder with anemia. Bone marrow examination revealed the involvement of 4.6% of GL and erythroblastopenia. A clonogenic assay of bone marrow cells revealed the decrease in erythroid colony formation in both CFU-E and BFU-E, but the number of erythroid colonies increased when CD8-positive cells were depleted from bone marrow cells and the number of erythroid colonies decreased again when CD8-positive GLs were added. The supernatant of cultured CD8-positive GLs had no inhibitory effect on CFU-E and BFU-E colony formation. These suggested that CD8-positive GLs suppressed the erythroid colony formation in this case. Treatment with 6,000 U/body of recombinant human erythropoietin (rh-Epo) subcutaneously three times a week was started and increased dose of 12,000 U/body of rh-Epo led to an increase in the hemoglobin level to 10.5 g/dl two months later. He has been treated with rh-Epo only.
一名54岁男性因疲劳入院。外周血细胞计数显示白细胞增多（9600/微升），其中包括76%的颗粒淋巴细胞（GLs），这些细胞表达CD2、3、8、16和HLA - DR，同时伴有贫血（血红蛋白8.1克/分升）。他被诊断为患有伴贫血的T细胞型颗粒淋巴细胞增殖性疾病。骨髓检查显示4.6%的GL受累且有红细胞生成减少。骨髓细胞克隆形成试验显示CFU - E和BFU - E中的红系集落形成均减少，但当从骨髓细胞中去除CD8阳性细胞时红系集落数量增加，而当加入CD8阳性GLs时红系集落数量再次减少。培养的CD8阳性GLs的上清液对CFU - E和BFU - E集落形成无抑制作用。这些提示在该病例中CD8阳性GLs抑制了红系集落形成。开始每周三次皮下注射6000单位/体的重组人促红细胞生成素（rh - Epo），将rh - Epo剂量增加至12000单位/体后，两个月后血红蛋白水平升至10.5克/分升。他一直仅接受rh - Epo治疗。
